Review



imagej software v1.51j8  (GraphPad Software Inc)


Bioz Verified Symbol GraphPad Software Inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    GraphPad Software Inc imagej software v1.51j8
    PMCA4b localization and function is impaired in the triple negative MDA-MB-231 cell line. a : Cell culture morphology analysis of the MCF-7 and MDA-MB-231 cell lines. Pictures were taken with a phase contrast microscope. Gray cell masks were made using the <t>ImageJ</t> software <t>v1.51j8</t> to emphasize the morphology of the cell cultures and cell-cell contacts. Scale bar: 30 μm. b : Subcellular localization of the PMCA4b protein in VPA-treated MCF-7 and MDA-MB-231 cells. MCF-7 cells were treated with 2 mM VPA, MDA-MB-231 cell were treated with 4 mM VPA for 4 days and immunostained with an anti-PMCA4b antibody (JA3). Images were taken by confocal microscopy. White arrows indicate PMCA4b protein in intracellular compartments. Scale bar: 30 μm. c : Relative PMCA4b expression in MCF-7 and MDA-MB-231 cells after a 4 day VPA treatment. Densitometric values of Western blots were normalized to the respective β-actin loading control levels and expressed as fold increase over the untreated controls. Bars represent mean ± SEM from two to four independent experiments. d : Ca 2+ signal measurements in VPA-treated GCaMP2-MCF-7 and GCaMP2-MDA-MB-231 cells. Cells were treated with 4 mM VPA for 4 days. Before the measurement, culture medium was replaced by HBSS supplemented with 2 mM Ca 2+ . Ca 2+ influx was triggered by 2 μM Ca 2+ ionophore A23187, and fluorescent signal of the GCaMP2 Ca 2+ sensor was followed by confocal imaging. F/F 0 values represent individual cells ( n = 14–32) from a representative experiment. e : Area under curve of the A23187-induced Ca 2+ transients. Bar graphs are means ± SEM of the individual cells ( n = 14–32). Significance between control and VPA-treated cells is denoted by *** ( P < 0.001); two-tailed unpaired t-test
    Imagej Software V1.51j8, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagej+software+v1%2E51j8/pmc06199715-87-5-13?v=GraphPad+Software+Inc
    Average 90 stars, based on 1 article reviews
    imagej software v1.51j8 - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Expression of calcium pumps is differentially regulated by histone deacetylase inhibitors and estrogen receptor alpha in breast cancer cells"

    Article Title: Expression of calcium pumps is differentially regulated by histone deacetylase inhibitors and estrogen receptor alpha in breast cancer cells

    Journal: BMC Cancer

    doi: 10.1186/s12885-018-4945-x

    PMCA4b localization and function is impaired in the triple negative MDA-MB-231 cell line. a : Cell culture morphology analysis of the MCF-7 and MDA-MB-231 cell lines. Pictures were taken with a phase contrast microscope. Gray cell masks were made using the ImageJ software v1.51j8 to emphasize the morphology of the cell cultures and cell-cell contacts. Scale bar: 30 μm. b : Subcellular localization of the PMCA4b protein in VPA-treated MCF-7 and MDA-MB-231 cells. MCF-7 cells were treated with 2 mM VPA, MDA-MB-231 cell were treated with 4 mM VPA for 4 days and immunostained with an anti-PMCA4b antibody (JA3). Images were taken by confocal microscopy. White arrows indicate PMCA4b protein in intracellular compartments. Scale bar: 30 μm. c : Relative PMCA4b expression in MCF-7 and MDA-MB-231 cells after a 4 day VPA treatment. Densitometric values of Western blots were normalized to the respective β-actin loading control levels and expressed as fold increase over the untreated controls. Bars represent mean ± SEM from two to four independent experiments. d : Ca 2+ signal measurements in VPA-treated GCaMP2-MCF-7 and GCaMP2-MDA-MB-231 cells. Cells were treated with 4 mM VPA for 4 days. Before the measurement, culture medium was replaced by HBSS supplemented with 2 mM Ca 2+ . Ca 2+ influx was triggered by 2 μM Ca 2+ ionophore A23187, and fluorescent signal of the GCaMP2 Ca 2+ sensor was followed by confocal imaging. F/F 0 values represent individual cells ( n = 14–32) from a representative experiment. e : Area under curve of the A23187-induced Ca 2+ transients. Bar graphs are means ± SEM of the individual cells ( n = 14–32). Significance between control and VPA-treated cells is denoted by *** ( P < 0.001); two-tailed unpaired t-test
    Figure Legend Snippet: PMCA4b localization and function is impaired in the triple negative MDA-MB-231 cell line. a : Cell culture morphology analysis of the MCF-7 and MDA-MB-231 cell lines. Pictures were taken with a phase contrast microscope. Gray cell masks were made using the ImageJ software v1.51j8 to emphasize the morphology of the cell cultures and cell-cell contacts. Scale bar: 30 μm. b : Subcellular localization of the PMCA4b protein in VPA-treated MCF-7 and MDA-MB-231 cells. MCF-7 cells were treated with 2 mM VPA, MDA-MB-231 cell were treated with 4 mM VPA for 4 days and immunostained with an anti-PMCA4b antibody (JA3). Images were taken by confocal microscopy. White arrows indicate PMCA4b protein in intracellular compartments. Scale bar: 30 μm. c : Relative PMCA4b expression in MCF-7 and MDA-MB-231 cells after a 4 day VPA treatment. Densitometric values of Western blots were normalized to the respective β-actin loading control levels and expressed as fold increase over the untreated controls. Bars represent mean ± SEM from two to four independent experiments. d : Ca 2+ signal measurements in VPA-treated GCaMP2-MCF-7 and GCaMP2-MDA-MB-231 cells. Cells were treated with 4 mM VPA for 4 days. Before the measurement, culture medium was replaced by HBSS supplemented with 2 mM Ca 2+ . Ca 2+ influx was triggered by 2 μM Ca 2+ ionophore A23187, and fluorescent signal of the GCaMP2 Ca 2+ sensor was followed by confocal imaging. F/F 0 values represent individual cells ( n = 14–32) from a representative experiment. e : Area under curve of the A23187-induced Ca 2+ transients. Bar graphs are means ± SEM of the individual cells ( n = 14–32). Significance between control and VPA-treated cells is denoted by *** ( P < 0.001); two-tailed unpaired t-test

    Techniques Used: Cell Culture, Microscopy, Software, Confocal Microscopy, Expressing, Western Blot, Imaging, Two Tailed Test



    Similar Products

    imagej software v1.51j8  (GraphPad Software Inc)
    90
    GraphPad Software Inc imagej software v1.51j8
    PMCA4b localization and function is impaired in the triple negative MDA-MB-231 cell line. a : Cell culture morphology analysis of the MCF-7 and MDA-MB-231 cell lines. Pictures were taken with a phase contrast microscope. Gray cell masks were made using the <t>ImageJ</t> software <t>v1.51j8</t> to emphasize the morphology of the cell cultures and cell-cell contacts. Scale bar: 30 μm. b : Subcellular localization of the PMCA4b protein in VPA-treated MCF-7 and MDA-MB-231 cells. MCF-7 cells were treated with 2 mM VPA, MDA-MB-231 cell were treated with 4 mM VPA for 4 days and immunostained with an anti-PMCA4b antibody (JA3). Images were taken by confocal microscopy. White arrows indicate PMCA4b protein in intracellular compartments. Scale bar: 30 μm. c : Relative PMCA4b expression in MCF-7 and MDA-MB-231 cells after a 4 day VPA treatment. Densitometric values of Western blots were normalized to the respective β-actin loading control levels and expressed as fold increase over the untreated controls. Bars represent mean ± SEM from two to four independent experiments. d : Ca 2+ signal measurements in VPA-treated GCaMP2-MCF-7 and GCaMP2-MDA-MB-231 cells. Cells were treated with 4 mM VPA for 4 days. Before the measurement, culture medium was replaced by HBSS supplemented with 2 mM Ca 2+ . Ca 2+ influx was triggered by 2 μM Ca 2+ ionophore A23187, and fluorescent signal of the GCaMP2 Ca 2+ sensor was followed by confocal imaging. F/F 0 values represent individual cells ( n = 14–32) from a representative experiment. e : Area under curve of the A23187-induced Ca 2+ transients. Bar graphs are means ± SEM of the individual cells ( n = 14–32). Significance between control and VPA-treated cells is denoted by *** ( P < 0.001); two-tailed unpaired t-test
    Imagej Software V1.51j8, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagej+software+v1%2E51j8/pmc06199715-87-5-13?v=GraphPad+Software+Inc
    Average 90 stars, based on 1 article reviews
    imagej software v1.51j8 - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    PMCA4b localization and function is impaired in the triple negative MDA-MB-231 cell line. a : Cell culture morphology analysis of the MCF-7 and MDA-MB-231 cell lines. Pictures were taken with a phase contrast microscope. Gray cell masks were made using the ImageJ software v1.51j8 to emphasize the morphology of the cell cultures and cell-cell contacts. Scale bar: 30 μm. b : Subcellular localization of the PMCA4b protein in VPA-treated MCF-7 and MDA-MB-231 cells. MCF-7 cells were treated with 2 mM VPA, MDA-MB-231 cell were treated with 4 mM VPA for 4 days and immunostained with an anti-PMCA4b antibody (JA3). Images were taken by confocal microscopy. White arrows indicate PMCA4b protein in intracellular compartments. Scale bar: 30 μm. c : Relative PMCA4b expression in MCF-7 and MDA-MB-231 cells after a 4 day VPA treatment. Densitometric values of Western blots were normalized to the respective β-actin loading control levels and expressed as fold increase over the untreated controls. Bars represent mean ± SEM from two to four independent experiments. d : Ca 2+ signal measurements in VPA-treated GCaMP2-MCF-7 and GCaMP2-MDA-MB-231 cells. Cells were treated with 4 mM VPA for 4 days. Before the measurement, culture medium was replaced by HBSS supplemented with 2 mM Ca 2+ . Ca 2+ influx was triggered by 2 μM Ca 2+ ionophore A23187, and fluorescent signal of the GCaMP2 Ca 2+ sensor was followed by confocal imaging. F/F 0 values represent individual cells ( n = 14–32) from a representative experiment. e : Area under curve of the A23187-induced Ca 2+ transients. Bar graphs are means ± SEM of the individual cells ( n = 14–32). Significance between control and VPA-treated cells is denoted by *** ( P < 0.001); two-tailed unpaired t-test

    Journal: BMC Cancer

    Article Title: Expression of calcium pumps is differentially regulated by histone deacetylase inhibitors and estrogen receptor alpha in breast cancer cells

    doi: 10.1186/s12885-018-4945-x

    Figure Lengend Snippet: PMCA4b localization and function is impaired in the triple negative MDA-MB-231 cell line. a : Cell culture morphology analysis of the MCF-7 and MDA-MB-231 cell lines. Pictures were taken with a phase contrast microscope. Gray cell masks were made using the ImageJ software v1.51j8 to emphasize the morphology of the cell cultures and cell-cell contacts. Scale bar: 30 μm. b : Subcellular localization of the PMCA4b protein in VPA-treated MCF-7 and MDA-MB-231 cells. MCF-7 cells were treated with 2 mM VPA, MDA-MB-231 cell were treated with 4 mM VPA for 4 days and immunostained with an anti-PMCA4b antibody (JA3). Images were taken by confocal microscopy. White arrows indicate PMCA4b protein in intracellular compartments. Scale bar: 30 μm. c : Relative PMCA4b expression in MCF-7 and MDA-MB-231 cells after a 4 day VPA treatment. Densitometric values of Western blots were normalized to the respective β-actin loading control levels and expressed as fold increase over the untreated controls. Bars represent mean ± SEM from two to four independent experiments. d : Ca 2+ signal measurements in VPA-treated GCaMP2-MCF-7 and GCaMP2-MDA-MB-231 cells. Cells were treated with 4 mM VPA for 4 days. Before the measurement, culture medium was replaced by HBSS supplemented with 2 mM Ca 2+ . Ca 2+ influx was triggered by 2 μM Ca 2+ ionophore A23187, and fluorescent signal of the GCaMP2 Ca 2+ sensor was followed by confocal imaging. F/F 0 values represent individual cells ( n = 14–32) from a representative experiment. e : Area under curve of the A23187-induced Ca 2+ transients. Bar graphs are means ± SEM of the individual cells ( n = 14–32). Significance between control and VPA-treated cells is denoted by *** ( P < 0.001); two-tailed unpaired t-test

    Article Snippet: Data were analyzed with the ImageJ software v1.51j8 and Prism 4 software v4.01 (GraphPad Software).

    Techniques: Cell Culture, Microscopy, Software, Confocal Microscopy, Expressing, Western Blot, Imaging, Two Tailed Test